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iscript cdna synthesis supermix reverse transcriptase  (Bio-Rad)


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    Structured Review

    Bio-Rad iscript cdna synthesis supermix reverse transcriptase
    Iscript Cdna Synthesis Supermix Reverse Transcriptase, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 6616 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iscript+reverse+transcriptase+supermix/IScript+C+DNA+Synthesis+Kit/bio_rxiv__2025__05__08__652960-56-8-14
    Average 99 stars, based on 6616 article reviews
    iscript cdna synthesis supermix reverse transcriptase - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Histone variant H3.3 mediates cGAS-STING pathway activation via telomere deprotection
    Article Snippet: Cells were lysed and harvested to extract total RNA using an RNA Mini Kit (Amersham). .. Reverse transcription was performed with iScript Reverse Transcriptase Supermix (Bio-Rad) to generate cDNA for quantitative analyses. .. Quantitative real-time PCR was performed using SYBR Green Select reagent (LifeTechnologies) to determine expression levels of target genes.

    Article Title: An injury-induced tissue niche shaped by mesenchymal plasticity coordinates the regenerative and disease response in the lung
    Article Snippet: Organoid diameters were measured at day 7 using FIJI (ImageJ). .. For qRT-PCR, RNA was isolated using the Direct-zol RNA Miniprep Plus Kit (Zymo). cDNA synthesis was performed using iScript reverse transcriptase supermix (Biorad). .. Gene expression was calculated relative to Rpl19 (“ L19 ”) within that sample and expressed as fold change over the average expression. qPCR was run on an Applied Biosystems QuantStudio 6 Real-Time PCR System (Thermo Fisher Scientific) with PowerUp SYBR Green Master Mix (Applied Biosystems).

    Article Title: Engineered Gold and Silica Nanoparticle-Incorporated Hydrogel Scaffolds for Human Stem Cell-Derived Cardiac Tissue Engineering
    Article Snippet: The RNA purity and concentration were then analyzed by 260/280 nm absorbance using Nanodrop one instrument (Thermo Scientific). .. Next, iScript Reverse Transcriptase Supermix (BioRad) was used to synthesize cDNA from the extracted RNA. .. Then, qPCR using the iTaq Universal SYBR Green Supermix (Bio-Rad) was used to analyze the synthesized cDNA.

    Article Title: Blood-derived lysophospholipid sustains hepatic phospholipids and fat storage necessary for hepatoprotection in overnutrition
    Article Snippet: The concentration of RNA was determined using Nanodrop (Thermo Fisher Scientific). .. Liver cDNA was synthesized from 1 μg of liver total RNA using iScript Reverse Transcriptase Supermix (170-8841, Bio-Rad) according to the manufacturer’s recommended protocol. .. Quantitative PCR (qPCR) was performed with the SensiFAST SYBR Hi-ROX Kit (BIO-92005, Bioline) using the CFX384 Touch Real-Time PCR Detection System (Bio-Rad). qPCR primers used in this study are listed in .

    Article Title: Cortactin Facilitates Malignant Transformation of Dysplastic Cells in Gastric Cancer Development
    Article Snippet: .. Total RNA was extracted from cortactin WT and KO Meta4 organoids using TRIzol (Thermo Fisher Scientific) with 0.2% glycogen. cDNA synthesis from the extracted total RNA was performed using iScript Reverse Transcriptase Supermix (Bio-Rad). qPCR was performed using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) and CFX96 Real-Time PCR Detection System (Bio-Rad) under manufacturer’s protocol. ..

    Article Title: Implications of population changes among the Arvicolinae (Rodentia, Mammalia) in El Mirón Cave (Cantabria, Spain) for the climate of the last c. 50,000 years
    Article Snippet: .. Liver cDNA was synthesized from 1 μg of liver total RNA using iScript Reverse Transcriptase Supermix (170-8841, Bio-Rad) according to the manufacturer’s recommended protocol. .. Quantitative PCR (qPCR) was performed with the SensiFAST SYBR Hi-ROX Kit (BIO-92005, Bioline) using the CFX384 Touch Real-Time PCR Detection System (Bio-Rad). qPCR primers used in this study are listed in Supplemental Data Set 1.

    Article Title: Pericytes require physiological oxygen tension to maintain phenotypic fidelity
    Article Snippet: .. RNA was quantified using a Nanodrop and 200 ng was used for reverse transcriptase cDNA synthesis using the iScript Reverse Transcriptase Supermix (1708841, BioRad). .. RT-qPCR was performed using cDNA diluted 1:30 in RNA free water, EvaGreen master mix (1725212, BioRad) and 10 μM primers.

    other:

    Article Title: Spatially Resolved Tumor Ecosystems and Cell States in Gastric Adenocarcinoma Progression and Evolution
    Article Snippet: SNU1967 cells were transfected with ON-TARGETplus Human siRNA SMARTpool targeting SOX9 (L-021507-00-0005) using Lipofectamine RNAiMAX (Thermo Fisher Scientific).

    Isolation:

    Article Title: An injury-induced tissue niche shaped by mesenchymal plasticity coordinates the regenerative and disease response in the lung
    Article Snippet: Organoid diameters were measured at day 7 using FIJI (ImageJ). .. For qRT-PCR, RNA was isolated using the Direct-zol RNA Miniprep Plus Kit (Zymo). cDNA synthesis was performed using iScript reverse transcriptase supermix (Biorad). .. Gene expression was calculated relative to Rpl19 (“ L19 ”) within that sample and expressed as fold change over the average expression. qPCR was run on an Applied Biosystems QuantStudio 6 Real-Time PCR System (Thermo Fisher Scientific) with PowerUp SYBR Green Master Mix (Applied Biosystems).

    cDNA Synthesis:

    Article Title: An injury-induced tissue niche shaped by mesenchymal plasticity coordinates the regenerative and disease response in the lung
    Article Snippet: Organoid diameters were measured at day 7 using FIJI (ImageJ). .. For qRT-PCR, RNA was isolated using the Direct-zol RNA Miniprep Plus Kit (Zymo). cDNA synthesis was performed using iScript reverse transcriptase supermix (Biorad). .. Gene expression was calculated relative to Rpl19 (“ L19 ”) within that sample and expressed as fold change over the average expression. qPCR was run on an Applied Biosystems QuantStudio 6 Real-Time PCR System (Thermo Fisher Scientific) with PowerUp SYBR Green Master Mix (Applied Biosystems).

    Article Title: Cortactin Facilitates Malignant Transformation of Dysplastic Cells in Gastric Cancer Development
    Article Snippet: .. Total RNA was extracted from cortactin WT and KO Meta4 organoids using TRIzol (Thermo Fisher Scientific) with 0.2% glycogen. cDNA synthesis from the extracted total RNA was performed using iScript Reverse Transcriptase Supermix (Bio-Rad). qPCR was performed using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) and CFX96 Real-Time PCR Detection System (Bio-Rad) under manufacturer’s protocol. ..

    Article Title: Pericytes require physiological oxygen tension to maintain phenotypic fidelity
    Article Snippet: .. RNA was quantified using a Nanodrop and 200 ng was used for reverse transcriptase cDNA synthesis using the iScript Reverse Transcriptase Supermix (1708841, BioRad). .. RT-qPCR was performed using cDNA diluted 1:30 in RNA free water, EvaGreen master mix (1725212, BioRad) and 10 μM primers.

    Synthesized:

    Article Title: Blood-derived lysophospholipid sustains hepatic phospholipids and fat storage necessary for hepatoprotection in overnutrition
    Article Snippet: The concentration of RNA was determined using Nanodrop (Thermo Fisher Scientific). .. Liver cDNA was synthesized from 1 μg of liver total RNA using iScript Reverse Transcriptase Supermix (170-8841, Bio-Rad) according to the manufacturer’s recommended protocol. .. Quantitative PCR (qPCR) was performed with the SensiFAST SYBR Hi-ROX Kit (BIO-92005, Bioline) using the CFX384 Touch Real-Time PCR Detection System (Bio-Rad). qPCR primers used in this study are listed in .

    Article Title: Implications of population changes among the Arvicolinae (Rodentia, Mammalia) in El Mirón Cave (Cantabria, Spain) for the climate of the last c. 50,000 years
    Article Snippet: .. Liver cDNA was synthesized from 1 μg of liver total RNA using iScript Reverse Transcriptase Supermix (170-8841, Bio-Rad) according to the manufacturer’s recommended protocol. .. Quantitative PCR (qPCR) was performed with the SensiFAST SYBR Hi-ROX Kit (BIO-92005, Bioline) using the CFX384 Touch Real-Time PCR Detection System (Bio-Rad). qPCR primers used in this study are listed in Supplemental Data Set 1.

    Real-time Polymerase Chain Reaction:

    Article Title: Cortactin Facilitates Malignant Transformation of Dysplastic Cells in Gastric Cancer Development
    Article Snippet: .. Total RNA was extracted from cortactin WT and KO Meta4 organoids using TRIzol (Thermo Fisher Scientific) with 0.2% glycogen. cDNA synthesis from the extracted total RNA was performed using iScript Reverse Transcriptase Supermix (Bio-Rad). qPCR was performed using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) and CFX96 Real-Time PCR Detection System (Bio-Rad) under manufacturer’s protocol. ..

    SYBR Green Assay:

    Article Title: Cortactin Facilitates Malignant Transformation of Dysplastic Cells in Gastric Cancer Development
    Article Snippet: .. Total RNA was extracted from cortactin WT and KO Meta4 organoids using TRIzol (Thermo Fisher Scientific) with 0.2% glycogen. cDNA synthesis from the extracted total RNA was performed using iScript Reverse Transcriptase Supermix (Bio-Rad). qPCR was performed using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) and CFX96 Real-Time PCR Detection System (Bio-Rad) under manufacturer’s protocol. ..



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